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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
500.00 | 2.558 | 2.478 |
250.00 | 1.476 | 1.397 |
125.00 | 0.853 | 0.773 |
62.50 | 0.558 | 0.478 |
31.25 | 0.324 | 0.245 |
15.63 | 0.218 | 0.138 |
7.82 | 0.174 | 0.094 |
0.00 | 0.080 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 88-101% | 93% |
EDTA plasma(n=5) | 94-103% | 99% |
Heparin plasma(n=5) | 90-105% | 98% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 89-101% | 85-98% | 88-99% | 85-101% |
EDTA plasma(n=5) | 95-106% | 96-108% | 90-105% | 86-102% |
Heparin plasma(n=5) | 91-104% | 88-102% | 92-100% | 89-105% |
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