Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
5000.00 | 2.781 | 2.716 |
2500.00 | 1.701 | 1.637 |
1250.00 | 0.906 | 0.842 |
625.00 | 0.508 | 0.443 |
312.50 | 0.319 | 0.254 |
156.25 | 0.177 | 0.113 |
78.13 | 0.120 | 0.056 |
0.00 | 0.064 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 88-99% | 94% |
EDTA plasma(n=5) | 102-110% | 104% |
Heparin plasma(n=5) | 98-104% | 101% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 93-106% | 88-102% | 85-101% | 96-107% |
EDTA plasma(n=5) | 90-103% | 94-108% | 90-106% | 98-105% |
Heparin plasma(n=5) | 91-100% | 98-107% | 92-108% | 93-102% |
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