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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
2500.00 | 2.126 | 2.022 |
1250.00 | 1.602 | 1.498 |
625.00 | 1.186 | 1.082 |
312.50 | 0.782 | 0.678 |
156.25 | 0.563 | 0.459 |
78.13 | 0.305 | 0.201 |
39.07 | 0.186 | 0.082 |
0.00 | 0.104 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 96-107% | 102% |
EDTA plasma(n=5) | 80-95% | 87% |
Heparin plasma(n=5) | 85-98% | 91% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-102% | 81-96% | 87-105% | 83-92% |
EDTA plasma(n=5) | 88-101% | 90-98% | 92-106% | 85-97% |
Heparin plasma(n=5) | 89-97% | 85-97% | 87-96% | 85-96% |
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