Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
40.00 | 2.179 | 2.094 |
20.00 | 1.602 | 1.517 |
10.00 | 1.182 | 1.097 |
5.00 | 0.896 | 0.811 |
2.50 | 0.554 | 0.469 |
1.25 | 0.321 | 0.236 |
0.63 | 0.190 | 0.105 |
0.00 | 0.085 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-95% | 87% |
EDTA plasma(n=5) | 92-106% | 100% |
Heparin plasma(n=5) | 87-99% | 93% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 83-92% | 91-96% | 85-93% | 95-101% |
EDTA plasma(n=5) | 85-94% | 87-101% | 89-99% | 85-95% |
Heparin plasma(n=5) | 86-94% | 93-102% | 89-97% | 85-94% |
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