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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1600.00 | 0.212 | |
800.00 | 0.385 | |
400.00 | 0.575 | |
200.00 | 0.895 | |
100.00 | 1.168 | |
50.00 | 1.585 | |
25.00 | 1.624 | |
0.00 | 2.256 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 92-105% | 98% |
EDTA plasma(n=5) | 83-95% | 89% |
Heparin plasma(n=5) | 87-99% | 93% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 89-97% | 86-102% | 94-106% | 85-97% |
EDTA plasma(n=5) | 96-101% | 85-92% | 79-96% | 89-101% |
Heparin plasma(n=5) | 86-102% | 87-96% | 89-98% | 83-103% |
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