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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1600.00 | 0.197 | |
800.00 | 0.385 | |
400.00 | 0.611 | |
200.00 | 0.812 | |
100.00 | 1.157 | |
50.00 | 1.583 | |
25.00 | 1.796 | |
0.00 | 2.213 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 95-107% | 101% |
EDTA plasma(n=5) | 78-95% | 86% |
Heparin plasma(n=5) | 82-95% | 88% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-97% | 87-96% | 82-95% | 92-105% |
EDTA plasma(n=5) | 90-99% | 95-106% | 87-101% | 82-97% |
Heparin plasma(n=5) | 87-95% | 85-97% | 89-101% | 85-97% |
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