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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
40.00 | 2.164 | 2.069 |
20.00 | 1.527 | 1.432 |
10.00 | 1.073 | 0.978 |
5.00 | 0.821 | 0.726 |
2.50 | 0.535 | 0.440 |
1.25 | 0.356 | 0.261 |
0.63 | 0.257 | 0.162 |
0.00 | 0.095 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 90-105% | 97% |
EDTA plasma(n=5) | 88-102% | 95% |
Heparin plasma(n=5) | 80-92% | 86% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 89-101% | 87-98% | 92-104% | 82-96% |
EDTA plasma(n=5) | 85-94% | 97-106% | 79-91% | 90-99% |
Heparin plasma(n=5) | 85-102% | 81-96% | 87-105% | 83-92% |
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