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一(yī)步法小(xiǎo)鼠25-羟基維生(shēng)素D(25-OH-D)酶聯免疫吸附檢測試劑盒
ELK052ES
規格: 價格:
48T ¥2660.00
96T ¥3800.00

Overview

Product name: EasyStep Mouse 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
Reactivity: Mouse
Alternative Names: 25 Hydroxy Vitamin D;
Assay Type: Competitive Inhibition
Sensitivity: 7.7 ng/mL
Standard: 5000 ng/mL
Detection Range: 78.13-5000 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 25-OH-D antigen, and the Mouse 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.

标準曲線

Concentration (ng/mL) OD Corrected OD
5000.00 0.175
2500.00 0.352
1250.00 0.577
625.00 0.833
312.50 1.152
156.25 1.565
78.13 1.789
0.00 2.132

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 25-OH-D and the recovery rates were calculated by comparing the measured value to the expected amount of 25-OH-D in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 78-96% 87%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25-OH-D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-102% 81-96% 93-106% 82-95%
EDTA plasma(n=5) 86-97% 83-96% 91-103% 96-105%
Heparin plasma(n=5) 85-100% 80-97% 81-92% 87-103%

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