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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
25.00 | 2.045 | 1.966 |
12.50 | 1.618 | 1.539 |
6.25 | 1.226 | 1.147 |
3.13 | 0.834 | 0.755 |
1.57 | 0.583 | 0.504 |
0.79 | 0.426 | 0.347 |
0.40 | 0.324 | 0.245 |
0.00 | 0.079 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-90% | 85% |
EDTA plasma(n=5) | 84-106% | 100% |
Heparin plasma(n=5) | 87-93% | 90% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 83-92% | 92-96% | 86-98% | 95-101% |
EDTA plasma(n=5) | 88-96% | 86-95% | 90-103% | 87-90% |
Heparin plasma(n=5) | 87-94% | 85-96% | 92-102% | 81-90% |
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