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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
20.00 | 1.948 | 1.867 |
10.00 | 1.502 | 1.421 |
5.00 | 1.116 | 1.035 |
2.50 | 0.705 | 0.624 |
1.25 | 0.468 | 0.387 |
0.63 | 0.308 | 0.227 |
0.32 | 0.226 | 0.145 |
0.00 | 0.081 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 98-104% | 102% |
EDTA plasma(n=5) | 95-107% | 101% |
Heparin plasma(n=5) | 96-104% | 100% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-98% | 81-96% | 87-98% | 92-103% |
EDTA plasma(n=5) | 89-101% | 79-92% | 82-93% | 83-97% |
Heparin plasma(n=5) | 82-103% | 81-91% | 85-101% | 81-90% |
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