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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
15.00 | 2.035 | 1.932 |
7.50 | 1.593 | 1.490 |
3.75 | 1.194 | 1.091 |
1.88 | 0.831 | 0.728 |
0.94 | 0.582 | 0.479 |
0.47 | 0.363 | 0.260 |
0.24 | 0.215 | 0.112 |
0.00 | 0.103 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 82-94% | 88% |
EDTA plasma(n=5) | 85-97% | 91% |
Heparin plasma(n=5) | 81-93% | 87% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 79-92% | 80-93% | 81-98% | 89-102% |
EDTA plasma(n=5) | 89-97% | 86-95% | 81-93% | 91-103% |
Heparin plasma(n=5) | 85-99% | 82-96% | 92-101% | 82-90% |
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