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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
4000.00 | 2.201 | 2.076 |
2000.00 | 1.717 | 1.592 |
1000.00 | 1.251 | 1.126 |
500.00 | 0.806 | 0.681 |
250.00 | 0.539 | 0.414 |
125.00 | 0.364 | 0.239 |
62.50 | 0.258 | 0.133 |
0.00 | 0.125 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 85-97% | 91% |
EDTA plasma(n=5) | 95-107% | 101% |
Heparin plasma(n=5) | 82-95% | 88% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 95-102% | 96-104% | 87-98% | 95-107% |
EDTA plasma(n=5) | 87-97% | 86-92% | 89-103% | 89-98% |
Heparin plasma(n=5) | 79-85% | 87-96% | 82-95% | 89-103% |
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