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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 1.972 | 1.876 |
500.00 | 1.527 | 1.431 |
250.00 | 1.107 | 1.011 |
125.00 | 0.821 | 0.725 |
62.50 | 0.535 | 0.439 |
31.25 | 0.342 | 0.246 |
15.63 | 0.221 | 0.125 |
0.00 | 0.096 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-92% | 86% |
EDTA plasma(n=5) | 85-97% | 91% |
Heparin plasma(n=5) | 91-99% | 95% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 95-102% | 85-96% | 90-98% | 82-93% |
EDTA plasma(n=5) | 89-97% | 85-92% | 95-103% | 87-93% |
Heparin plasma(n=5) | 89-102% | 97-103% | 88-96% | 95-101% |
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