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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
10.00 | 1.946 | 1.857 |
5.00 | 1.507 | 1.418 |
2.50 | 1.128 | 1.039 |
1.25 | 0.928 | 0.839 |
0.63 | 0.563 | 0.474 |
0.32 | 0.342 | 0.253 |
0.16 | 0.196 | 0.107 |
0.00 | 0.089 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 82-94% | 88% |
EDTA plasma(n=5) | 97-107% | 102% |
Heparin plasma(n=5) | 87-99% | 93% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 87-95% | 78-91% | 97-103% | 95-101% |
EDTA plasma(n=5) | 89-103% | 79-92% | 88-97% | 85-97% |
Heparin plasma(n=5) | 95-102% | 97-105% | 87-94% | 93-101% |
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