Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.179 | 2.075 |
500.00 | 1.828 | 1.724 |
250.00 | 1.436 | 1.332 |
125.00 | 1.036 | 0.932 |
62.50 | 0.794 | 0.690 |
31.25 | 0.423 | 0.319 |
15.63 | 0.228 | 0.124 |
0.00 | 0.104 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 85-97% | 91% |
EDTA plasma(n=5) | 80-94% | 87% |
Heparin plasma(n=5) | 91-105% | 98% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 95-102% | 93-101% | 81-89% | 85-99% |
EDTA plasma(n=5) | 88-97% | 79-92% | 81-97% | 87-95% |
Heparin plasma(n=5) | 85-94% | 96-105% | 87-101% | 82-90% |
關注微信公衆号
微信掃一(yī)掃立即咨詢
微信掃一(yī)掃立即咨詢