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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
50.00 | 1.846 | 1.760 |
25.00 | 1.527 | 1.441 |
12.50 | 1.034 | 0.948 |
6.25 | 0.675 | 0.589 |
3.13 | 0.424 | 0.338 |
1.57 | 0.312 | 0.226 |
0.79 | 0.208 | 0.122 |
0.00 | 0.086 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 86-98% | 92% |
EDTA plasma(n=5) | 95-107% | 101% |
Heparin plasma(n=5) | 82-95% | 88% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-92% | 97-103% | 87-98% | 95-102% |
EDTA plasma(n=5) | 98-103% | 86-92% | 87-93% | 93-102% |
Heparin plasma(n=5) | 85-94% | 97-106% | 92-101% | 82-90% |
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