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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
10.00 | 2.105 | 2.036 |
5.00 | 1.436 | 1.367 |
2.50 | 0.947 | 0.878 |
1.25 | 0.438 | 0.369 |
0.63 | 0.296 | 0.227 |
0.32 | 0.187 | 0.118 |
0.16 | 0.142 | 0.073 |
0.00 | 0.069 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 85-97% | 91% |
EDTA plasma(n=5) | 79-97% | 88% |
Heparin plasma(n=5) | 81-97% | 89% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-92% | 80-96% | 87-98% | 95-102% |
EDTA plasma(n=5) | 98-105% | 86-92% | 87-93% | 95-103% |
Heparin plasma(n=5) | 91-98% | 79-93% | 92-101% | 82-90% |
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