微信掃一(yī)掃立即咨詢
Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 1.898 | 1.827 |
500.00 | 1.209 | 1.138 |
250.00 | 0.674 | 0.603 |
125.00 | 0.361 | 0.290 |
62.50 | 0.180 | 0.109 |
31.25 | 0.172 | 0.101 |
15.63 | 0.152 | 0.081 |
0.00 | 0.071 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 81-96% | 88% |
EDTA plasma(n=5) | 78-94% | 86% |
Heparin plasma(n=5) | 90-103% | 96% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-92% | 89-103% | 97-105% | 91-99% |
EDTA plasma(n=5) | 88-102% | 86-92% | 81-93% | 89-104% |
Heparin plasma(n=5) | 95-104% | 79-92% | 82-95% | 86-97% |
關注微信公衆号
微信掃一(yī)掃立即咨詢
微信掃一(yī)掃立即咨詢