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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
20.00 | 2.231 | 2.176 |
10.00 | 1.771 | 1.716 |
5.00 | 1.037 | 0.982 |
2.50 | 0.671 | 0.616 |
1.25 | 0.351 | 0.296 |
0.63 | 0.193 | 0.138 |
0.32 | 0.120 | 0.065 |
0.00 | 0.055 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 85-97% | 91% |
EDTA plasma(n=5) | 80-95% | 87% |
Heparin plasma(n=5) | 89-103% | 96% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-92% | 87-103% | 90-98% | 95-102% |
EDTA plasma(n=5) | 88-97% | 86-92% | 95-103% | 89-97% |
Heparin plasma(n=5) | 79-94% | 87-96% | 82-95% | 89-102% |
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