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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
20.00 | 2.242 | 2.186 |
10.00 | 1.697 | 1.641 |
5.00 | 1.151 | 1.095 |
2.50 | 0.674 | 0.618 |
1.25 | 0.424 | 0.368 |
0.63 | 0.206 | 0.150 |
0.32 | 0.137 | 0.081 |
0.00 | 0.056 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 78-90% | 84% |
EDTA plasma(n=5) | 82-97% | 89% |
Heparin plasma(n=5) | 91-105% | 98% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 79-92% | 85-96% | 80-98% | 95-102% |
EDTA plasma(n=5) | 88-97% | 86-102% | 91-103% | 89-97% |
Heparin plasma(n=5) | 85-102% | 87-96% | 82-90% | 92-101% |
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