Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.168 | 2.110 |
500.00 | 1.319 | 1.261 |
250.00 | 0.773 | 0.715 |
125.00 | 0.418 | 0.360 |
62.50 | 0.251 | 0.193 |
31.25 | 0.185 | 0.127 |
15.63 | 0.122 | 0.064 |
0.00 | 0.058 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 78-90% | 84% |
EDTA plasma(n=5) | 84-97% | 90% |
Heparin plasma(n=5) | 87-95% | 91% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 83-96% | 81-94% | 86-98% | 92-101% |
EDTA plasma(n=5) | 85-96% | 86-95% | 90-102% | 81-90% |
Heparin plasma(n=5) | 87-95% | 83-96% | 92-102% | 82-93% |
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