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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.270 | 2.201 |
500.00 | 1.529 | 1.460 |
250.00 | 0.885 | 0.816 |
125.00 | 0.495 | 0.426 |
62.50 | 0.266 | 0.197 |
31.25 | 0.163 | 0.094 |
15.63 | 0.118 | 0.049 |
0.00 | 0.069 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 82-97% | 90% |
EDTA plasma(n=5) | 88-106% | 97% |
Heparin plasma(n=5) | 87-99% | 93% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 81-92% | 92-103% | 87-98% | 90-101% |
EDTA plasma(n=5) | 83-96% | 86-95% | 90-102% | 81-93% |
Heparin plasma(n=5) | 83-94% | 85-96% | 92-103% | 86-97% |
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