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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
4000.00 | 2.402 | 2.341 |
2000.00 | 1.822 | 1.761 |
1000.00 | 1.148 | 1.087 |
500.00 | 0.780 | 0.719 |
250.00 | 0.479 | 0.418 |
125.00 | 0.269 | 0.208 |
62.50 | 0.191 | 0.130 |
0.00 | 0.061 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 95-106% | 101% |
EDTA plasma(n=5) | 84-99% | 91% |
Heparin plasma(n=5) | 87-102% | 94% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 86-93% | 91-105% | 87-98% | 99-105% |
EDTA plasma(n=5) | 89-99% | 83-103% | 98-105% | 96-101% |
Heparin plasma(n=5) | 85-105% | 84-98% | 95-102% | 88-102% |
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