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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
40.00 | 2.166 | 2.089 |
20.00 | 1.569 | 1.492 |
10.00 | 0.870 | 0.793 |
5.00 | 0.499 | 0.422 |
2.50 | 0.270 | 0.193 |
1.25 | 0.172 | 0.095 |
0.63 | 0.119 | 0.042 |
0.00 | 0.077 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 78-90% | 84% |
EDTA plasma(n=5) | 84-97% | 90% |
Heparin plasma(n=5) | 90-105% | 97% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 89-102% | 79-94% | 83-96% | 85-106% |
EDTA plasma(n=5) | 88-97% | 90-99% | 85-97% | 79-101% |
Heparin plasma(n=5) | 78-94% | 97-105% | 88-101% | 82-96% |
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