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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
100.00 | 2.218 | 2.058 |
50.00 | 1.964 | 1.804 |
25.00 | 1.528 | 1.368 |
12.50 | 0.899 | 0.739 |
6.25 | 0.577 | 0.417 |
3.13 | 0.351 | 0.191 |
1.57 | 0.264 | 0.104 |
0.00 | 0.160 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 79-91% | 85% |
EDTA plasma(n=5) | 81-97% | 89% |
Heparin plasma(n=5) | 84-99% | 91% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-92% | 88-96% | 87-98% | 95-102% |
EDTA plasma(n=5) | 98-105% | 96-103% | 87-95% | 83-92% |
Heparin plasma(n=5) | 95-104% | 87-96% | 89-101% | 97-105% |
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