Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
20.00 | 2.082 | 1.895 |
10.00 | 1.761 | 1.574 |
5.00 | 1.251 | 1.064 |
2.50 | 0.862 | 0.675 |
1.25 | 0.503 | 0.316 |
0.63 | 0.401 | 0.214 |
0.32 | 0.224 | 0.037 |
0.00 | 0.187 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 78-90% | 84% |
EDTA plasma(n=5) | 89-107% | 98% |
Heparin plasma(n=5) | 87-99% | 93% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-92% | 79-96% | 87-103% | 90-99% |
EDTA plasma(n=5) | 88-97% | 96-105% | 91-103% | 89-97% |
Heparin plasma(n=5) | 85-94% | 87-96% | 79-95% | 95-102% |
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