人補體(tǐ)成分(fēn)3(C3)酶聯免疫吸附檢測試劑盒-武汉义森科技有限责任公司

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人補體(tǐ)成分(fēn)3(C3)酶聯免疫吸附檢測試劑盒

ELK1059

規格：	價格：
48T	￥1680.00
96T	￥2400.00

下(xià)載說明 ①

Overview

Product name:	Human C3(Complement Component 3) ELISA Kit
Reactivity:	Human
Alternative Names:	ASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein
Assay Type:	Sandwich
Sensitivity:	2.8 ng/mL
Standard:	500 ng/mL
Detection Range:	7.82-500 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;Infection immunity;Immune molecule;
Uniprot ID:	P01024
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C3 in the samples is then determined by comparing the OD of the samples to the standard curve.

标準曲線

Concentration (ng/mL)	OD	Corrected OD
500.00	1.997	1.898
250.00	1.586	1.487
125.00	1.277	1.178
62.50	0.935	0.836
31.25	0.596	0.497
15.63	0.379	0.280
7.82	0.197	0.098
0.00	0.099	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant C3 and the recovery rates were calculated by comparing the measured value to the expected amount of C3 in samples.

Matrix	Recovery range	Average
serum(n=5)	90-104%	97%
EDTA plasma(n=5)	80-97%	88%
Heparin plasma(n=5)	87-99%	93%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.