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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.135 | 2.063 |
500.00 | 1.562 | 1.490 |
250.00 | 0.997 | 0.925 |
125.00 | 0.682 | 0.610 |
62.50 | 0.395 | 0.323 |
31.25 | 0.273 | 0.201 |
15.63 | 0.135 | 0.063 |
0.00 | 0.072 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 78-96% | 87% |
EDTA plasma(n=5) | 78-92% | 85% |
Heparin plasma(n=5) | 80-95% | 87% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 86-94% | 85-95% | 89-99% | 85-95% |
EDTA plasma(n=5) | 89-97% | 85-94% | 79-96% | 85-99% |
Heparin plasma(n=5) | 79-93% | 89-97% | 85-94% | 87-101% |
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