Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
500.00 | 2.126 | 2.040 |
250.00 | 1.673 | 1.587 |
125.00 | 1.128 | 1.042 |
62.50 | 0.901 | 0.815 |
31.25 | 0.614 | 0.528 |
15.63 | 0.403 | 0.317 |
7.82 | 0.256 | 0.170 |
0.00 | 0.086 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 86-97% | 92% |
EDTA plasma(n=5) | 84-103% | 96% |
Heparin plasma(n=5) | 82-96% | 87% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 86-93% | 93-101% | 85-98% | 90-101% |
EDTA plasma(n=5) | 91-99% | 83-97% | 87-105% | 87-99% |
Heparin plasma(n=5) | 85-90% | 83-96% | 95-102% | 88-95% |
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