Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
500.00 | 2.153 | 2.068 |
250.00 | 1.628 | 1.543 |
125.00 | 1.243 | 1.158 |
62.50 | 0.948 | 0.863 |
31.25 | 0.507 | 0.422 |
15.63 | 0.357 | 0.272 |
7.82 | 0.215 | 0.130 |
0.00 | 0.085 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 81-95% | 88% |
EDTA plasma(n=5) | 87-99% | 93% |
Heparin plasma(n=5) | 87-99% | 93% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 89-97% | 85-94% | 79-96% | 85-99% |
EDTA plasma(n=5) | 86-98% | 91-103% | 85-97% | 87-96% |
Heparin plasma(n=5) | 87-95% | 85-97% | 89-101% | 85-97% |
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