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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.214 | 2.086 |
500.00 | 1.784 | 1.656 |
250.00 | 1.243 | 1.115 |
125.00 | 0.872 | 0.744 |
62.50 | 0.546 | 0.418 |
31.25 | 0.325 | 0.197 |
15.63 | 0.240 | 0.112 |
0.00 | 0.128 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 83-95% | 89% |
EDTA plasma(n=5) | 88-102% | 95% |
Heparin plasma(n=5) | 79-97% | 88% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 87-96% | 91-101% | 87-98% | 89-103% |
EDTA plasma(n=5) | 84-94% | 85-103% | 94-105% | 92-101% |
Heparin plasma(n=5) | 88-104% | 84-98% | 95-105% | 88-102% |
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