Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 1.933 | 1.840 |
500.00 | 1.522 | 1.429 |
250.00 | 1.123 | 1.030 |
125.00 | 0.896 | 0.803 |
62.50 | 0.587 | 0.494 |
31.25 | 0.325 | 0.232 |
15.63 | 0.235 | 0.142 |
0.00 | 0.093 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-96% | 88% |
EDTA plasma(n=5) | 80-95% | 87% |
Heparin plasma(n=5) | 83-95% | 89% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 88-98% | 87-103% | 89-97% | 85-94% |
EDTA plasma(n=5) | 95-103% | 79-97% | 93-102% | 87-103% |
Heparin plasma(n=5) | 85-93% | 79-96% | 93-106% | 87-99% |
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