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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.126 | 2.038 |
500.00 | 1.563 | 1.475 |
250.00 | 1.035 | 0.947 |
125.00 | 0.752 | 0.664 |
62.50 | 0.509 | 0.421 |
31.25 | 0.324 | 0.236 |
15.63 | 0.226 | 0.138 |
0.00 | 0.088 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 95-107% | 101% |
EDTA plasma(n=5) | 96-104% | 100% |
Heparin plasma(n=5) | 98-104% | 102% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 87-96% | 92-105% | 89-102% | 92-101% |
EDTA plasma(n=5) | 88-102% | 87-102% | 85-96% | 87-101% |
Heparin plasma(n=5) | 88-101% | 85-93% | 89-97% | 85-94% |
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