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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
200.00 | 2.075 | 1.987 |
100.00 | 1.502 | 1.414 |
50.00 | 1.174 | 1.086 |
25.00 | 0.963 | 0.875 |
12.50 | 0.529 | 0.441 |
6.25 | 0.357 | 0.269 |
3.13 | 0.202 | 0.114 |
0.00 | 0.088 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 82-94% | 88% |
EDTA plasma(n=5) | 85-97% | 91% |
Heparin plasma(n=5) | 81-93% | 87% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 86-95% | 93-103% | 87-98% | 85-96% |
EDTA plasma(n=5) | 88-102% | 87-102% | 85-96% | 87-101% |
Heparin plasma(n=5) | 86-93% | 93-101% | 86-97% | 90-101% |
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