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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
10.00 | 2.201 | 2.115 |
5.00 | 1.593 | 1.507 |
2.50 | 1.113 | 1.027 |
1.25 | 0.847 | 0.761 |
0.63 | 0.487 | 0.401 |
0.32 | 0.339 | 0.253 |
0.16 | 0.208 | 0.122 |
0.00 | 0.086 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-95% | 87% |
EDTA plasma(n=5) | 80-93% | 86% |
Heparin plasma(n=5) | 95-107% | 101% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-107% | 84-98% | 95-103% | 86-98% |
EDTA plasma(n=5) | 86-93% | 91-105% | 87-98% | 99-102% |
Heparin plasma(n=5) | 89-99% | 83-101% | 93-104% | 96-101% |
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