小(xiǎo)鼠胰島素樣生(shēng)長因子2(IGF2)酶聯免疫吸附檢測試劑盒-武汉义森科技有限责任公司

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小(xiǎo)鼠胰島素樣生(shēng)長因子2(IGF2)酶聯免疫吸附檢測試劑盒

ELK2258

規格：	價格：
48T	￥1960.00
96T	￥2800.00

下(xià)載說明 ①

Overview

Product name:	Mouse IGF2(Insulin Like Growth Factor 2) ELISA Kit
Reactivity:	Mouse
Alternative Names:	INSIGF; IGF-II; Somatomedin A; Preptin; Insulin-like growth factor II Ala-25 Del
Assay Type:	Sandwich
Sensitivity:	6.8 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Tumor immunity;Endocrinology;
Uniprot ID:	P09535
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IGF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IGF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IGF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IGF2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标準曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.328	2.220
500.00	1.745	1.637
250.00	1.219	1.111
125.00	0.907	0.799
62.50	0.509	0.401
31.25	0.386	0.278
15.63	0.226	0.118
0.00	0.108	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IGF2 and the recovery rates were calculated by comparing the measured value to the expected amount of IGF2 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	86-99%	92%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.