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Concentration (μmol/L) | OD | Corrected OD |
---|---|---|
10.00 | 2.256 | 2.168 |
5.00 | 1.628 | 1.540 |
2.50 | 1.216 | 1.128 |
1.25 | 0.752 | 0.664 |
0.63 | 0.509 | 0.421 |
0.32 | 0.265 | 0.177 |
0.16 | 0.196 | 0.108 |
0.00 | 0.088 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 83-97% | 90% |
EDTA plasma(n=5) | 87-95% | 91% |
Heparin plasma(n=5) | 81-95% | 88% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 88-97% | 86-92% | 81-93% | 93-104% |
EDTA plasma(n=5) | 87-94% | 91-104% | 95-107% | 82-96% |
Heparin plasma(n=5) | 81-94% | 93-107% | 93-102% | 91-105% |
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