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Concentration (mIU/mL) | OD | Corrected OD |
---|---|---|
100.00 | 1.952 | 1.884 |
50.00 | 1.602 | 1.534 |
25.00 | 1.211 | 1.143 |
12.50 | 0.892 | 0.824 |
6.25 | 0.593 | 0.525 |
3.13 | 0.335 | 0.267 |
1.57 | 0.241 | 0.173 |
0.00 | 0.068 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 81-95% | 88% |
EDTA plasma(n=5) | 80-95% | 87% |
Heparin plasma(n=5) | 87-99% | 85% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 89-102% | 97-103% | 97-104% | 82-97% |
EDTA plasma(n=5) | 85-94% | 93-101% | 87-98% | 89-102% |
Heparin plasma(n=5) | 98-102% | 96-102% | 89-103% | 89-96% |
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