Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
20.00 | 2.388 | 2.303 |
10.00 | 1.736 | 1.651 |
5.00 | 1.169 | 1.084 |
2.50 | 0.828 | 0.743 |
1.25 | 0.521 | 0.436 |
0.63 | 0.324 | 0.239 |
0.32 | 0.235 | 0.150 |
0.00 | 0.085 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-97% | 88% |
EDTA plasma(n=5) | 90-105% | 97% |
Heparin plasma(n=5) | 87-95% | 91% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 81-98% | 78-90% | 91-99% | 97-103% |
EDTA plasma(n=5) | 82-94% | 82-96% | 88-101% | 90-99% |
Heparin plasma(n=5) | 87-98% | 95-102% | 86-97% | 88-95% |
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